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Our Abpromise guarantee covers the use of ab188696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The activity of ab188696 was measured at 2.0 RFU/min/μg using an enzyme concentration of 18 μM and a Ub-AMC concentration of 4 μM in 100 mM HEPES, pH 8.5, 10 mM DTT, and 5% Glycerol at 25°C. USP14 activity is greatly stimulated in the presence of 19S proteasome.
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 1.19% HEPES, 0.29% Sodium chloride, 0.03% EDTA, 0.08% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab188696 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"