The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The activity of ab188696 was measured at 2.0 RFU/min/μg using an enzyme concentration of 18 μM and a Ub-AMC concentration of 4 μM in 100 mM HEPES, pH 8.5, 10 mM DTT, and 5% Glycerol at 25°C. USP14 activity is greatly stimulated in the presence of 19S proteasome.
>95% by SDS-PAGE . Purified under reducing conditions and visualized by Coomassie Blue.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Deubiquitinating enzyme 14
tRNA guanine transglycosylase
tRNA guanine transglycosylase 60 kD subunit
Ubiquitin carboxyl terminal hydrolase 14
Ubiquitin carboxyl-terminal hydrolase 14
Ubiquitin specific peptidase 14
Ubiquitin specific processing protease 14
Ubiquitin specific protease 14
Ubiquitin thiolesterase 14
Ubiquitin-specific-processing protease 14
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs).
Belongs to the peptidase C19 family. USP14/UBP6 subfamily. Contains 1 ubiquitin-like domain.