The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Typical enzyme concentration to support hydrolysis of substrates in vitro ranges from 10-100 nM depending on conditions and substrate. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Ubiquitin specific protease 5 (ubiquitin isopeptidase T)
Ubiquitin thioesterase 5
Ubiquitin thiolesterase 5
Ubiquitin-specific-processing protease 5
FunctionCleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
Sequence similaritiesBelongs to the peptidase C19 family. Contains 2 UBA domains. Contains 1 UBP-type zinc finger.
DomainThe UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.
Post-translational modificationsThe N-terminus is blocked.