Recombinant Human USP5 protein (Chemical Free) (ab80732)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab80732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Additional notes

    Typical enzyme concentration to support hydrolysis of substrates in vitro ranges from 10-100 nM depending on conditions and substrate. Pre-incubation (15 min) with 10 mM DTT is recommended to achieve maximum activity.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM HEPES, 150mM Sodium chloride, 1mM DTT, pH 8.0

General Info

  • Alternative names
    • Deubiquitinating enzyme 5
    • Isopeptidase T
    • ISOT
    • ISOT 1
    • Ubiquitin carboxyl-terminal hydrolase 5
    • Ubiquitin isopeptidase T
    • Ubiquitin specific peptidase 5
    • Ubiquitin specific peptidase 5 (isopeptidase T)
    • Ubiquitin specific processing protease 5
    • Ubiquitin specific protease 5 (isopeptidase T)
    • Ubiquitin specific protease 5 (ubiquitin isopeptidase T)
    • Ubiquitin thioesterase 5
    • Ubiquitin thiolesterase 5
    • Ubiquitin-specific-processing protease 5
    • UBP5_HUMAN
    • Usp5
    see all
  • Function
    Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
  • Sequence similarities
    Belongs to the peptidase C19 family.
    Contains 2 UBA domains.
    Contains 1 UBP-type zinc finger.
  • Domain
    The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Information by UniProt

References

ab80732 has not yet been referenced specifically in any publications.

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