The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThe ED50 for this effect is < 4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell.
Endotoxin level< 1.000 Eu/µg
% SDS-PAGE. purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 10% Glycerol, 20mM Tris HCl, pH 8
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Vascular endothelial growth factor A
Vascular permeability factor
RelevanceVEGFA is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145.
In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF 121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.
Recombinant human VEGF 121B protein images
SDS-PAGE - VEGF 121B protein (Human) (ab86935)
15% SDS Page analysis of 3ug ab86935
Functional Studies - VEGF 121B protein (Human) (ab86935)
NIH/3T3 cell proliferation was determined by BrdU incorporation method at 405 nm. This test was performed duplicate.N.C. : negative control [only 0.1% FBS]
P.C. : positive control [10% FBS]
References for Recombinant human VEGF 121B protein (ab86935)
has not yet been referenced specifically in any publications.
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