Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA
    • Amino acids
      22 to 207

Specifications

Our Abpromise guarantee covers the use of ab87052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml.
    Activity Assay:
    1. Cell line: NIH-3T3 (Mus musculus/Mouse embryonic fibroblast)
    2. Maintenance Condition: RPMI 1640 containing 10% FBS
    3. Assay medium: serum free RPMI 1640
    4. Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul)
    5. Incubation time : 24 hr (after sample treatment)
    6. Concentration range: 10pg/ml - 10ug/ml
    7. Detection method : BrdU assay
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab87052 was purified using conventional chromatography techniques
  • Form
    Liquid
  • Additional notes
    Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method)
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Vascular endothelial growth factor B
    • Vascular endothelial growth factor-related factor
    • VEGF B
    • VEGF related factor
    • VEGF-B
    • VEGF-related factor
    • Vegfb
    • VEGFB_HUMAN
    • VEGFL
    • VRF
    see all
  • Function
    Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
  • Tissue specificity
    Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
  • Sequence similarities
    Belongs to the PDGF/VEGF growth factor family.
  • Post-translational
    modifications
    VEGF-B186 is O-glycosylated.
  • Cellular localization
    Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
  • Information by UniProt

Recombinant human VEGFB protein images

References for Recombinant human VEGFB protein (ab87052)

ab87052 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab87052.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up