Recombinant human VEGFD protein (ab125747)

Overview

Description

  • NatureRecombinant
  • SourceHEK 293 cells
  • Amino Acid Sequence
    • AccessionO43915
    • SpeciesHuman
    • Molecular weight12 kDa
    • Amino acids93 to 201
    • TagsHis tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe ED50 of 3-4ng/ml is measured by its ability to stimulate the proliferation of Human microvascular endothelial cells (HMVECs).
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    ab125747 was lyophilized after extensive dialysis against PBS.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Store under Desiccating conditions.

    Constituent: 99% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionIt is recommended to reconstitute the ab125747 in sterile ultra pure water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Upon reconstitution ab125747 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

General Info

  • Alternative names
    • c-fos induced growth factor
    • c-fos induced growth factor (vascular endothelial growth factor D)
    • c-fos-induced growth factor
    • FIGF
    • Vascular endothelial growth factor D
    • Vascular endothelial growth factor D precursor
    • VEGF D
    • VEGF-D
    • VEGFD
    • VEGFD_HUMAN
    see all
  • FunctionGrowth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
  • Tissue specificityHighly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.
  • Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
  • Post-translational
    modifications
    Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant human VEGFD protein (ab125747)

ab125747 has not yet been referenced specifically in any publications.

Product Wall

Unfortunately, we have not tested this product's ability to induce phosphorylation of VEGFR2 and so we do not know whether it is capable of doing so.

We have only tested it's ability to stimulate the proliferation of Human microvascular endot...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"