Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      Q9YMF3
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHDSTKTWSEVFENSGCKPRPMVFRVHDEHPE LTSQRFNPPCVTLMRCGGCCNDESLECVPTEEANVTMQLMGASVSGGNGM QHLSFVEHKKCDCKPPLTTTPPTTTRPPRRRR
    • Amino acids
      21 to 81
    • Tags
      His tag N-Terminus
    • Additional sequence information
      Recombinant VEGFE with an N-terminal His-tag sequence and a thrombin cleavage site. GenBank accession No. AF106020.

Specifications

Our Abpromise guarantee covers the use of ab53679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGFE has been determined to be in the range of 5 - 20 ng/ml. Specific activity: 2 x 105 units/mg.

  • Applications

    SDS-PAGE

  • Purity
    > 90 % SDS-PAGE.
    Purity: > 90%, by SDS-PAGE and visualised by silver stain. Endotoxin level: < 0.1 ng per ug of VEGFE.
  • Form
    Lyophilised
  • Additional notes
    Like VEGFA, VEGFE was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGFA, VEGFE can not bind to VEGF receptor-1 (Flt-1). Furthermore VEGFE can also not bind to VEGF receptor-3 (FLT-4). Therefore VEGFE is a potent angiogenic factor selectively binding to VEGF receptor –2/KDR.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: PBS, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    The lyophilised VEGFE is soluble in water and most aqueous buffers. The lyophilised VEGFE should be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.

General Info

  • Relevance
    Based on sequence similarity to VEGFA, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGFA and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGFE proteins (Dehio et al., 1999; Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGFE is distinct from the previously described VEGFs but most closely related to VEGFA.

References

ab53679 has not yet been referenced specifically in any publications.

Publishing research using ab53679? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

There are currently no Customer reviews or Questions for ab53679.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up