Recombinant Human Visfatin protein (ab109137)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      52 kDa including tags
    • Amino acids
      1 to 491
    • Tags
      DDDDK tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab109137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Additional notes
    Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

  • Reconstitution
    Reconstitute in distilled water

General Info

  • Alternative names
    • 1110035O14Rik
    • AI314458
    • AI480535
    • DKFZP666B131
    • EC 2.4.2.12
    • MGC117256
    • NAmPRTase
    • Nampt
    • NAMPT_HUMAN
    • Nicotinamide phosphoribosyltransferase
    • PBEF
    • PBEF 1
    • PBEF1
    • Pre B cell colony enhancing factor
    • Pre B cell colony enhancing factor 1
    • Pre B cell enhancing factor
    • Pre-B cell-enhancing factor
    • Pre-B-cell colony-enhancing factor 1
    • VF
    • Visfatin
    see all
  • Function
    Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
  • Tissue specificity
    Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
  • Pathway
    Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
  • Sequence similarities
    Belongs to the NAPRTase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • Dimer formation of recombinant PBEF (ab109137).
    Purified ab109137 was separated by SDS-PAGE and Western blot analysis was performed using rabbit anti-Nampt polyclonal antibody. In the absence of DTT ab109137 formed a homodimer, a homomultimer.

References

ab109137 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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