Recombinant Human Vitamin D Receptor protein (ab82068)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids1 to 427

Specifications

Our Abpromise guarantee covers the use of ab82068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Other

    Western blot

    EMSA

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

General Info

  • Alternative names
    • 1
    • 1 25 dihydroxyvitamin D3 receptor
    • 1,25 dihydroxyvitamin D3 receptor
    • 1,25-@dihydroxyvitamin D3 receptor
    • 25-dihydroxyvitamin D3 receptor
    • Member 1
    • NR1I1
    • Nuclear receptor subfamily 1 group I member 1
    • PPP1R163
    • Protein phosphatase 1, regulatory subunit 163
    • VDR
    • VDR_HUMAN
    • Vitamin D (1,25- dihydroxyvitamin D3) receptor
    • Vitamin D hormone receptor
    • Vitamin D nuclear receptor variant 1
    • Vitamin D receptor
    • Vitamin D3 receptor
    see all
  • FunctionNuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
  • Involvement in diseaseDefects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt

Recombinant Human Vitamin D Receptor protein images

  • Anti-Vitamin D Receptor antibody (ab82566) at 1 µg/ml + Recombinant Human Vitamin D Receptor protein (ab82068) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

References for Recombinant Human Vitamin D Receptor protein (ab82068)

ab82068 has not yet been referenced specifically in any publications.

Product Wall

Apologies for the delay in replying to your question. The unit size is 5 ug and the concentration 0.5 ug/ul, therefore you will receive 10 ul. We suggest diluting the protein in the storage buffer, then aliquot and freeze. The storage buffer is as f...

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Our product, ab82068, can be used in competitive binding assays. This protein can be diluted in whatever buffer is appropriate for use in your assay; however I would like to point out that we do not recommend diluting this antibody before freezing it. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"