Overview

  • Product name
    Recombinant Human VPS24 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MGSSHHHHHHSSGLVPRGSHRKAVSKLYASKAHMNSVLMGMKNQLAVLRV AGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFES MDDQEEMEEEMGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQRE EEKVKRSVKDAAKKGQKDVCIVLAKEMIRSAEMEIDRILFEITAGALGKA PSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS
    • Molecular weight
      27 kDa including tags
    • Amino acids
      1 to 222
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab109845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity
    > 90 % SDS-PAGE.
    ab109845 is purified by using conventional chromatography techniques.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20% Glycerol, 0.316% Tris HCl, 0.058% Sodium chloride, 0.0154% DTT, pH 8.0

General Info

  • Alternative names
    • VPS 24
    • VPS24
    • CGI 149
    • CGI149 protein
    • Charged multivesicular body protein 3
    • CHMP3
    • CHMP3_HUMAN
    • Chromatin modifying protein 3
    • Chromatin-modifying protein 3
    • HGNC:29865
    • hVps24
    • NEDF
    • Neuroendocrine differentiation factor
    • Vacuolar protein sorting 24 (yeast)
    • Vacuolar protein sorting 24 homolog (S. cerevisiae)
    • Vacuolar protein sorting associated protein 24
    • Vacuolar protein sorting-associated protein 24
    • vps24
    see all
  • Function
    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Required for sorting/trafficking of EGF receptor.
  • Tissue specificity
    Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the SNF7 family.
  • Domain
    The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • Cellular localization
    Cytoplasm > cytosol. Membrane. Endosome. Late endosome membrane. Localizes to the midbody of dividing cells.
  • Information by UniProt

Images

  • 15% SDS-PAGE showing ab109845 at approximately 27.2 kDa (3µg).

References

ab109845 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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