Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ99986
    • SpeciesHuman
    • Molecular weight71 kDa including tags
    • Amino acids1 to 396

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe specific activity of ab125555 was determined to be 2 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 95 % Densitometry.
    Purity was determined to be >95% by densitometry.
  • FormLiquid
  • Additional notes

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • MGC117401
    • MGC138280
    • MGC142070
    • PCH1
    • PCH1A
    • Serine/threonine protein kinase VRK1
    • Serine/threonine-protein kinase VRK1
    • Vaccinia related kinase 1
    • Vaccinia virus B1R related kinase 1
    • Vaccinia-related kinase 1
    • VRK1
    • VRK1_HUMAN
    see all
  • FunctionSerine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.
  • Tissue specificityWidely expressed. Highly expressed in fetal liver, testis and thymus.
  • Involvement in diseaseDefects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1) [MIM:607596]; also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA).
  • Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53.
  • Cellular localizationNucleus. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
  • Information by UniProt

Recombinant human VRK1 protein images

  • SDS-PAGE analysis of ab125555.
  • Sample Kinase Assay showing the Specific Activity of ab125555 to be 2 nmol/min/mg.

References for Recombinant human VRK1 protein (ab125555)

ab125555 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"