Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf21 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MAAHHRQNTAGRRKVQVSYVIRDEVEKYNRNGVNALQLDPALNRLFTAGR DSIIRIWSVNQHKQDPYIASMEHHTDWVNDIVLCCNGKTLISASSDTTVK VWNAHKGFCMSTLRTHKDYVKALAYAKDKELVASAGLDRQIFLWDVNTLT ALTASNNTVTTSSLSGNKDSIYSLAMNQLGTIIVSGSTEKVLRVWDPRTC AKLMKLKGHTDNVKALLLNRDGTQCLSGSSDGTIRLWSLGQQRCIATYRV HDEGVWALQVNDAFTHVYSGGRDRKIYCTDLRNPDIRVLICEEKAPVLKM ELDRSADPPPAIWVATTKSTVNKWTLKGIHNFRASGDYDNDCTNPITPLC TQPDQVIKGGASIIQCHILNDKRHILTKDTNNNVAYWDVLKACKVEDLGK VDFEDEIKKRFKMVYVPNWFSVDLKTGMLTITLDESDCFAAWVSAKDAGF SSPDGSDPKLNLGGLLLQALLEYWPRTHVNPMDEEENEVNHVNGEQENRV QKGNGYFQVPPHTPVIFGEAGGRTLFRLLCRDSGGETESMLLNETVPQWV IDITVDKNMPKFNKIPFYLQPHASSGAKTLKKDRLSASDMLQVRKVMEHV YEKIINLDNESQTTSSSNNEKPGEQEKEEDIAVLAEEKIELLCQDQVLDP NMDLRTVKHFIWKSGGDLTLHYRQKST
    • Molecular weight
      76 kDa
    • Amino acids
      1 to 677
    • Tags
      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab188697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    ab188697 is an activator of Ubiquitin-­specific deconjugating enzymes USP1, USP12, and USP46. It activates USP1 at 1:1 stoichiometry in in vitro deconjugation reactions utilizing Ubiquitin-AMC.

  • Applications

    SDS-PAGE

    Functional Studies

  • Purity
    >90% by SDS-PAGE.
    Purified under reducing conditions and visualized by Coomassie Blue.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 10% Glycerol, 0.03% TCEP

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • p80
    • UAF 1
    • UAF1
    • USP1 associated factor 1
    • USP1-associated factor 1
    • WD repeat containing protein 48
    • WD repeat domain 48
    • WD repeat endosomal protein
    • WD repeat-containing protein 48
    • WDR 48
    • WDR48
    • WDR48_HUMAN
    see all
  • Function
    Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 by enhancing the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself. Also activates deubiquitinating activity of complexes containing USP12 and USP46, respectively. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the WD repeat WDR48 family.
    Contains 8 WD repeats.
  • Domain
    N-terminal WD region interacts with TIP and C-terminal region mediates lysosomal localization. The WD repeats are required for the interaction with deubiquitinating enzymes USP1, USP12 and USP46.
  • Cellular localization
    Nucleus. Cytoplasm. Lysosome. Mainly cytoplasmic. In case of infection by papillomavirus HPV11, translocates to the nucleus via its interaction with papillomavirus HPV11.
  • Information by UniProt

References

ab188697 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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