The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
< 0.100 Eu/µg
% SDS-PAGE. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Note: Do not raise to neutral pH. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working
aliquots at -20oC to -80oC.
CCN family member 5
Connective tissue growth factor like protein
Connective tissue growth factor related protein 58
Connective tissue growth factor-like protein
Connective tissue growth factor-related protein 58
Wnt 1 signaling pathway protein 2
WNT1 inducible signaling pathway protein 2
WNT1-inducible-signaling pathway protein 2
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
Expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.
Belongs to the CCN family. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain.
Anti-WISP2 antibody (ab38317) at 1/100 dilution + Recombinant human WISP2 protein (ab50040) at 0.01 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
References for Recombinant human WISP2 protein (ab50040)
This product has been referenced in:
Grünberg JR et al. Overexpressing the novel autocrine/endocrine adipokine WISP2 induces hyperplasia of the heart, white and brown adipose tissues and prevents insulin resistance. Sci Rep7:43515 (2017).
Read more (PubMed: 28240264) »