Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF

Specifications

Our Abpromise guarantee covers the use of ab50040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 95 % SDS-PAGE.
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Note: Do not raise to neutral pH. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.

General Info

  • Alternative names
    • CCN family member 5
    • CCN5
    • Connective tissue growth factor like protein
    • Connective tissue growth factor related protein 58
    • Connective tissue growth factor-like protein
    • Connective tissue growth factor-related protein 58
    • CT58
    • CTGF L
    • CTGF-L
    • WISP 2
    • WISP-2
    • Wisp2
    • WISP2_HUMAN
    • Wnt 1 signaling pathway protein 2
    • WNT1 inducible signaling pathway protein 2
    • WNT1-inducible-signaling pathway protein 2
    see all
  • Function
    May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
  • Tissue specificity
    Expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.
  • Sequence similarities
    Belongs to the CCN family.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • Anti-WISP2 antibody (ab38317) at 1/100 dilution + Recombinant human WISP2 protein (ab50040) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References

This product has been referenced in:
  • Grünberg JR  et al. Overexpressing the novel autocrine/endocrine adipokine WISP2 induces hyperplasia of the heart, white and brown adipose tissues and prevents insulin resistance. Sci Rep 7:43515 (2017). Read more (PubMed: 28240264) »

See 1 Publication for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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