• NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • AccessionQ9H4A3
    • SpeciesHuman
    • Molecular weight67 kDa including tags
    • Amino acids181 to 507


Our Abpromise guarantee covers the use of ab105196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe Specific activity of ab105196 was determined to be 30 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies


  • Purity> 95 % SDS-PAGE.
    Purity was determined to be >95% by densitometry.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Erythrocyte 65 kDa protein
    • HSAN2
    • HSN2
    • hWNK1
    • KDP
    • KIAA0344
    • Kinase deficient protein
    • MGC163339
    • MGC163341
    • p65
    • PRKWNK1
    • Prostate derived sterile 20 like kinase
    • Protein kinase lysine deficient 1
    • Protein kinase lysine-deficient 1
    • Protein kinase with no lysine 1
    • PSK
    • Serine/threonine protein kinase WNK1
    • Serine/threonine-protein kinase WNK1
    • With no K
    • WNK lysine deficient protein kinase 1
    • WNK1
    • WNK1_HUMAN
    see all
  • FunctionSerine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
  • Tissue specificityWidely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
  • Involvement in diseasePseudohypoaldosteronism 2C
    Neuropathy, hereditary sensory and autonomic, 2A
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
  • Cellular localizationCytoplasm.
  • Information by UniProt

Recombinant human WNK1 protein images

  • The Specific activity of ab105196 was determined to be 30 nmol/min/mg.
  • SDS-PAGE showing ab105196 at approximately 67kDa.

References for Recombinant human WNK1 protein (ab105196)

ab105196 has not yet been referenced specifically in any publications.

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