Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP04628
    • SpeciesHuman
    • SequenceANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL
    • Molecular weight38 kDa
    • Amino acids28 to 370
    • Additional sequence informationThis protein is a non-glycosylated full length mature protein that does not contain the signal peptide from aa 1 - 27.

Specifications

Our Abpromise guarantee covers the use of ab84080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 95 % SDS-PAGE.
    Purity is greater than 98% by SDS-PAGE gel and HPLC analyses.
  • FormLyophilised
  • Additional notesEndotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in water to a concentration of 0.1 – 1 mg/ml. ab84080 reconstituted is stable for at least 6 months when stored in working aliquots with a carrier protein such as 0.1% BSA at -20oC. Avoid repeated freeze/thaw cycles.

General Info

  • Alternative names
    • BMND16
    • INT1
    • OI15
    • oncogene Int1
    • Proto oncogene protein Wnt 1
    • Proto-oncogene Int-1 homolog
    • Proto-oncogene Wnt-1
    • Wingless type MMTV integration site family member 1
    • wingless-type MMTV integration site family, member 1 (oncogene INT1)
    • Wnt 1
    • wnt1
    • WNT1_HUMAN
    see all
  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt

References for Recombinant human Wnt1 protein (ab84080)

This product has been referenced in:
  • Xie W  et al. Sox2 modulates Lef-1 expression during airway submucosal gland development. Am J Physiol Lung Cell Mol Physiol 306:L645-60 (2014). Mouse . Read more (PubMed: 24487391) »
  • Cai WY  et al. The Wnt-ß-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion. J Cell Sci 126:2877-89 (2013). Read more (PubMed: 23613467) »

See all 8 Publications for this product

Product Wall

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We have validated ab109822 for use with the biotinylated ab84243 and will guarantee these antibodies for use as an sELISA pair. For the positive control, I would recommend using the full length protein, such as ab...

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Thank you for your enquiry regarding ab84080. For ab84080, the initial reconstitution should be performed in water to a concentration of 0.1-1.0mg/ml. So, the maximum reconstitution concentration is 1mg/ml (1000ug/ml). I hope this information he...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"