Recombinant Human Xanthine Oxidase protein (ab159820)

Overview

  • Product name
    Recombinant Human Xanthine Oxidase protein
  • Protein length
    Protein fragment

Description

  • Nature
    Recombinant
  • Source
    Wheat germ
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      GSIPIEFRVSLLRDCPNKKAIYASKAVGEPPLFLAASIFFAIKDAIRAAR AQHTGNNVKELFRLDSPATPEKIRNACVDKFTTLCVTGVPENCKPWSVR
    • Amino acids
      1234 to 1332
    • Tags
      proprietary tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab159820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

  • Form
    Liquid
  • Additional notes
    Protein concentration is above or equal to 0.05 mg/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names
    • Xanthine dehydrogenase
    • Xanthine dehydrogenase/oxidase
    • Xanthine oxidase
    • Xanthine oxidoreductase
    • XD
    • XDH
    • XDH_HUMAN
    • xdha
    • XO
    • xor
    see all
  • Function
    Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
  • Tissue specificity
    Detected in milk (at protein level).
  • Involvement in disease
    Defects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol.
  • Sequence similarities
    Belongs to the xanthine dehydrogenase family.
    Contains 1 2Fe-2S ferredoxin-type domain.
    Contains 1 FAD-binding PCMH-type domain.
  • Post-translational
    modifications
    Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
    Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
  • Cellular localization
    Cytoplasm. Peroxisome. Secreted.
  • Information by UniProt

Images

  • ab159820 on a 12.5% SDS-PAGE stained with Coomassie Blue.

References

ab159820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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