Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab89852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab89852 was determined to be 62 nmol/min/mg
  • Applications

    SDS-PAGE

    Western blot

    Functional Studies

  • Form
    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AZK
    • Cervical cancer suppressor gene 4 protein
    • HCCS 4
    • HCCS-4
    • HCCS4
    • Human cervical cancer suppressor gene 4 protein
    • Leucine zipper and sterile alpha motif containing kinase
    • Leucine zipper- and sterile alpha motif-containing kinase
    • Mitogen activated protein kinase kinase kinase MLT
    • Mitogen-activated protein kinase kinase kinase MLT
    • Mixed lineage kinase 7
    • Mixed lineage kinase related kinase
    • Mixed lineage kinase related kinase MRK beta
    • Mixed lineage kinase with a leucine zipper and a sterile alpha motif
    • Mixed lineage kinase-related kinase
    • MLK 7
    • MLK like mitogen activated protein triple kinase
    • MLK related kinase
    • MLK-like mitogen-activated protein triple kinase
    • MLK-related kinase
    • MLK7
    • Mlklak
    • MLT
    • MLTK_HUMAN
    • MRK
    • Sterile alpha motif and leucine zipper containing kinase AZK
    • Sterile alpha motif- and leucine zipper-containing kinase AZK
    • Zak
    see all
  • Function
    Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.
  • Tissue specificity
    Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates to the nucleus upon ultraviolet B irradiation.
  • Information by UniProt

Images

  • SDS-PAGE showing ab89852 at approximately 82kDa
  • Kinase Assay demonstrating specific activity of ab89852

References

ab89852 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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