Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab79644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: 113 nmol/min/mg.
  • Applications

    Functional Studies

    SDS-PAGE

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • 70 kDa zeta associated protein
    • 70 kDa zeta-associated protein
    • EC 2.7.10.2
    • FLJ17670
    • FLJ17679
    • Selective T cell defect
    • SRK
    • STD
    • Syk related tyrosine kinase
    • Syk-related tyrosine kinase
    • Truncated ZAP kinase
    • Tyrosine protein kinase ZAP70
    • Tyrosine-protein kinase ZAP-70
    • TZK
    • ZAP 70
    • ZAP70
    • ZAP70_HUMAN
    • Zeta chain associated protein kinase 70kD
    • Zeta chain associated protein kinase 70kDa
    • Zeta chain associated protein kinase 70kDa isoform 1
    • Zeta chain associated protein kinase 70kDa isoform 2
    • Zeta chain of T cell receptor associated protein kinase 70
    • Zeta chain TCR associated protein kinase 70kD
    • Zeta chain TCR associated protein kinase 70kDa
    see all
  • Function
    Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
  • Tissue specificity
    Expressed in T- and natural killer cells.
  • Involvement in disease
    Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.
  • Domain
    The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
  • Cellular localization
    Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt

Images

  • ab79644 on SDS-PAGE, Molecular Weight 96 kDa
    10% SDS-PAGE, Coomassie staining
    Lane 1: Protein marker
    Lane 2: ab79644
  • Kinase assay - Specific Activity 113 nmol/min/mg

References

ab79644 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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