Recombinant IL4 protein (ab87385)

Overview

Description

  • Nature
    Recombinant
  • Source
    Yeast
  • Amino Acid Sequence
    • Sequence
      GCKYDITLQE IIKTLNNLTD GKGKNSCMEL TVADAFAGPK NTDGKEICRA AKVLQQLYKR HDRSLIKECL SGLDRNLKGM ANGTCCTVNE AKKSTLKDFL ERLKTIMKEK YSKC

Specifications

Our Abpromise guarantee covers the use of ab87385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The biological activity of recombinant equine IL4 was measured in a cell proliferation assay using the human TF1 cell line. The ED50 for this effect is typically 40 - 60 ng/mL.
  • Applications

    Cellular Activation

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    ab87385 is purified by Ion-exchange chromatography.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    Preservative: None
    Constituents: 10% Trehalose, 1.0X PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

General Info

  • Alternative names
    • B cell growth factor 1
    • B cell IgG differentiation factor
    • B Cell Stimulatory Factor 1
    • B-cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF-1
    • BSF1
    • IGG1 induction factor
    • IL 4
    • IL-4
    • IL4
    • IL4_HUMAN
    • Il4e12
    • Interleukin 4
    • Interleukin 4 variant 2
    • Interleukin 4, isoform 1
    • Interleukin-4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • Function
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
  • Involvement in disease
    Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities
    Belongs to the IL-4/IL-13 family.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab87385 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. The ED50 is an expression of activity at 50% of its maximum response. Specific activity is just another way of conveying the activity of the protein. To calculate a specific activity, divide 1 x 106 by the ED50 in ng/ml...

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Thank you for contacting us. As per your original requirements we have few products that will be suitable for your needs. ab73186; The ED50 is 0.05-0.2 ng/ml; amount is 10ug; price is Euro 360; packaging and delivery charges 42 Euros. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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