Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant Influenza A Virus Hemagglutinin H7 protein -
Purity
> 80 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Predicted molecular weight
62 kDa -
Additional sequence information
From virus strain A/Anhui/1/2013 (H7N9).
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Specifications
Our Abpromise guarantee covers the use of ab190421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
Recombinant hemagglutinin glycoprotein provides a control protein for immunoassays and a tool to enhance Orthomyxovirus research.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituent: 99% Tris buffered saline
Contains 0.01% non-ionic detergent.
General Info
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Alternative names
- HA7
- Hemagglutinin 7
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Relevance
Haemagglutinin (HA) is one of two main surface fusion glycoproteins embedded in the envelope of influenza viruses, the other being neuraminidase (NA). There are sixteen known HA subtypes (H1-H16) and nine NA subtypes (N1-N9), which together are used to classify influenza viruses (e.g. H5N1). The antigenic variations in HA and NA enable the virus to evade host antibodies made to previous influenza strains, accounting for recurrent influenza epidemics.
Images
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Hemagglutination with turkey red blood cells: Hemagglutinin Titer 1:2048
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SDS-PAGE analysis of 0.4 µg ab190421 under reducing conditions.
The theoretical molecular weight of the protein is ~62 kDa, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.
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Anti-H7 NY mouse antiserum at 1/100 dilution +
Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421) at 0.38 µg
Secondary
Anti-mouse AP conjugateQuality control testing demonstrates strong detection of GP null under reduced conditions when using anti-H7 influenza antiserum.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab190421 has been referenced in 1 publication.
- Novoseltseva AA et al. Structural and Functional Aspects of G-Quadruplex Aptamers Which Bind a Broad Range of Influenza A Viruses. Biomolecules 10:N/A (2020). PubMed: 31936820