The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Recombinant hemagglutinin glycoprotein provides a control protein for immunoassays and a tool to enhance Orthomyxovirus research.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituent: 99% Tris buffered saline
Contains 0.01% non-ionic detergent.
Haemagglutinin (HA) is one of two main surface fusion glycoproteins embedded in the envelope of influenza viruses, the other being neuraminidase (NA). There are sixteen known HA subtypes (H1-H16) and nine NA subtypes (N1-N9), which together are used to classify influenza viruses (e.g. H5N1). The antigenic variations in HA and NA enable the virus to evade host antibodies made to previous influenza strains, accounting for recurrent influenza epidemics.
Functional Studies - Influenza A Virus Hemagglutinin H7 full length protein (ab190421)
Hemagglutination with turkey red blood cells: Hemagglutinin Titer 1:2048
SDS-PAGE - Influenza A Virus Hemagglutinin H7 full length protein (ab190421)
SDS-PAGE analysis of 0.4 µg ab190421 under reducing conditions.
The theoretical molecular weight of the protein is ~62 kDa, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.
Western blot - Influenza A Virus Hemagglutinin H7 full length protein (ab190421)
Anti-H7 NY mouse antiserum at 1/100 dilution + Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421) at 0.38 µg
Secondary Anti-mouse AP conjugate
Quality control testing demonstrates strong detection of GP null under reduced conditions when using anti-H7 influenza antiserum.
has not yet been referenced specifically in any publications.
Publishing research using ab190421? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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