Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli

Specifications

Our Abpromise guarantee covers the use of ab9601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Cell differentiation

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 0.100 Eu/µg
  • FormLyophilised
  • Additional notesThe ED50, determined by the dose-dependent stimulation of of thymidine uptake by KGF-responsive BaF3 cells, is < 10.0 ng/ml, corresponding to a specific activity of > 1.0 x 105 units/mg.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    PBS, pH 7.0, no preservative, sterile filtered

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • FGF 7
    • FGF-7
    • Fgf7
    • FGF7_HUMAN
    • Fibroblast growth factor 7
    • HBGF 7
    • HBGF-7
    • HBGF7
    • Heparin binding growth factor 7
    • Heparin-binding growth factor 7
    • Keratinocyte growth factor
    • KGF
    see all
  • FunctionGrowth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
  • Tissue specificityEpithelial cell.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Cellular localizationSecreted.
  • Information by UniProt

Recombinant KGF protein images

  • Definitive endoderm derived from culturing human embryonic stem cells (HUES4) with Inhibin beta a (ab50051) and Wnt3a (ab81484) was further differentiated to gut tube endoderm as described in Kroon et al, Nat Biotechnol. (2008). KGF (ab9601) was added to the media to direct differentiation towards gut tube endoderm. This image was taken 3 days after addition of KGF, where the gut tube endoderm marker TCF2 (ab59118 in green) is present. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.

References for Recombinant KGF protein (ab9601)

ab9601 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your interesting enquiry regaring ab9601. The peptide/proteinproduct we supply are often used as controls for western blotting. However, there are quite a few references you may be interested in which I have listed below. With regards...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"