Recombinant Listeriolysin (LLO) protein (ab83345)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • Amino acids60 to 529

Specifications

Our Abpromise guarantee covers the use of ab83345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityHemolytic activity: 1.25x106 HU/mg. 2 mM DTT could be use to reactivate the toxin.
  • Applications

    Functional Studies

    SDS-PAGE

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 5% Glycerol, 0.5M Sodium chloride, 50mM Sodium phosphate, 2.7mM Potassium chloride, 1mM DTT, 1mM EDTA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • hly
    • hlyA
    • lisA
    • LLO
    • Thiol activated cytolysin
    see all
  • RelevanceListeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion. Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol. LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.

Recombinant Listeriolysin (LLO) protein images

  • ab83345 by SDS Page, MW 52.5 kDa

References for Recombinant Listeriolysin (LLO) protein (ab83345)

ab83345 has not yet been referenced specifically in any publications.

Product Wall

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