The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Cytotoxicity can be detected in Human neutrophils when used in combination with LukE in a concentration range of 12.5-800 nM.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituent: 100% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
SDS-PAGE - (ab190423)
SDS-PAGE analysis of ab190423: 1 µg (lane 1) and 5 µg (lane 2).
Western blot - (ab190423)
Anti-LukF-PV polyclonal antibody at 0.5 µg/ml + Recombinant LukD protein (ab190423) at 0.1 µg
Secondary Anti-rabbit IgG-HRP conjugate
Functional Studies - (ab190423)
Toxin Functionality: Human promyelocytic leukemia cell line HL60 was differentiated into neutrophils by treatment with DMSO. Neutrophils were incubated with serial dilutions of rLukE and ab190423 alone (Open circles and open squares, respectively) or equimolar concentration of both proteins (Closed circles) for 3 hours at 37°C with 5% CO2 and 95% humidity. Cellular viability was determined by adding XTT and incubation for additional 16 hours. Cells were centrifuged and the OD determined in the supernatants at 470nm. EC50 was found to be 95.1 nM of each toxin subunit.
has not yet been referenced specifically in any publications.
Publishing research using ab190423? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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