Recombinant mouse AK 1 protein (Active) (ab227415)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, MS, SDS-PAGE
Description
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Product name
Recombinant mouse AK 1 protein (Active) -
Biological activity
Specific activity is > 150 units/mg. One unit will convert 2.0 µmoles of ADP to ATP + AMP per minute at pH 7.5 at 37°C.
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Purity
> 90 % SDS-PAGE.
ab227415 was purified by using conventional chromatography techniques. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMGCCVSSEPQEEGGRKTGEKLKKAKII FVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSERGKKLSAIM EKGELVPLDTVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFEQKIGQ PTLLLYVDAGAETMTQRLLKRGETSGRVDDNEETIKKRLETYYNATEPVI SFYDKRGIVRKVNAEGTVDTVFSEVCTYLDSLK -
Predicted molecular weight
26 kDa including tags -
Amino acids
1 to 210 -
Tags
His tag N-Terminus -
Additional sequence information
NP_067490
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab227415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Additional notes
Previously labelled as Adenylate Kinase 1.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.02% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Adenylate kinase 1
- Adenylate kinase isoenzyme 1
- Adenylate kinase soluble
see all -
Function
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. -
Involvement in disease
Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency [MIM:612631]. -
Sequence similarities
Belongs to the adenylate kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227415 has not yet been referenced specifically in any publications.