Recombinant mouse AHA1 protein (ab111143)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, WB
Description
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Product name
Recombinant mouse AHA1 protein
See all AHA1 proteins and peptides -
Biological activity
2uM ab111143 generated a 9-fold ATPase activation of 2uM Hsp90 (His-tagged Hsp90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees celcius (this is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
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Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Predicted molecular weight
38 kDa -
Amino acids
1 to 338 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab111143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Constituents: 0.002% DTT, 0.378% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Activator of 90 kDa heat shock protein ATPase homolog 1
- Activator of heat shock 90kDa protein ATPase homolog 1
- AHA 1
see all -
Function
Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking. -
Tissue specificity
Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta. -
Sequence similarities
Belongs to the AHA1 family. -
Cellular localization
Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab111143 has not yet been referenced specifically in any publications.