Recombinant mouse Aspartate Aminotransferase protein (His tag) (ab201889)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
Description
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Product name
Recombinant mouse Aspartate Aminotransferase protein (His tag)
See all Aspartate Aminotransferase proteins and peptides -
Biological activity
Specific activity is > 80 units/mg, and is defined as the amount of enzyme that converts 1.0 μmole of α-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25°C.
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Purity
> 95 % SDS-PAGE.
ab201889 was purified using conventional chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSMAPPSVFAQVPQAPPVLVFKLTADFRD DPDPRKVNLGVGAYRTDESQPWVLPVVRKVEQKIANDNSLNHEYLPILGL AEFRSCASRLVLGDNSPAIRENRVGGVQSLGGTGALRIGADFLGRWYNGT DNKNTPIYVSSPTWENHNAVFSAAGFKDIRPYCYWDAEKRGLDLQGFLND LENAPEFSIFVLHACAHNPTGTDPTPEQWKQIAAVMQRRFLFPFFDSAYQ GFASGDLEKDAWAIRYFVSEGFELFCAQSFSKNFGLYNERVGNLTVVGKE SDSVLRVLSQMEKIVRITWSNPPAQGARIVAATLSDPELFKEWKGNVKTM ADRILTMRSELRARLEALKTPGTWSHITEQIGMFSFTGLNPKQVEYLVNE KHIYLLPSGRINMCGLTTKNLDYVATSIHEAVTKIQ -
Predicted molecular weight
49 kDa including tags -
Amino acids
1 to 413 -
Tags
His tag N-Terminus -
Additional sequence information
NCBI Accession No.: AAH02057
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Associated products
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Related Buffer
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab201889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 88% PBS, 10% Glycerol (glycerin, glycerine), 0.02% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- AATC_HUMAN
- Aspartate aminotransferase
- Aspartate aminotransferase 1
see all -
Function
Plays a key role in amino acid metabolism. -
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. -
Cellular localization
Cytoplasm. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab201889 has not yet been referenced specifically in any publications.