Recombinant Mouse Dkk3 protein (ab207108)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Mouse Dkk3 protein
See all Dkk3 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
PSPTVTWTPAEPGPALNYPQEEATLNEMFREVEELMEDTQHKLRSAVEEM EAEEAAAKTSSEVNLASLPPNYHNETSTETRVGNNTVHVHQEVHKITNNQ SGQVVFSETVITSVGDEEGKRSHECIIDEDCGPTRYCQFSSFKYTCQPCR DQQMLCTRDSECCGDQLCAWGHCTQKATKGGNGTICDNQRDCQPGLCCAF QRGLLFPVCTPLPVEGELCHDPTSQLLDLITWELEPEGALDRCPCASGLL CQPHSHSLVYMCKPAFVGSHDHSEESQLPREAPDEYEDVGFIGEVRQELE DLERSLAQEMAFEGPAPVESLGGEEEI -
Predicted molecular weight
37 kDa including tags -
Amino acids
23 to 349 -
Tags
His tag C-Terminus -
Additional sequence information
This product is the mature full length protein from aa 23 to 349. The signal peptide is not included (NP_056629.1).
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab207108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution. -
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- Dickkopf 3
- Dickkopf 3 homolog
- Dickkopf 3 homolog (Xenopus laevis)
see all -
Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
Tissue specificity
Highest expression in heart, brain, and spinal cord. -
Sequence similarities
Belongs to the dickkopf family. -
Domain
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab207108 has not yet been referenced specifically in any publications.