Recombinant Mouse DLK protein (Fc fragment) (ab108574)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Mouse
    • Molecular weight
      75 kDa
    • Amino acids
      1 to 305
    • Additional sequence information
      Fused with the Fc region of Human IgG1 at the C-terminus.

Specifications

Our Abpromise guarantee covers the use of ab108574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells.
  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 90 % SDS-PAGE.
    ab108574 is 0.2µm filtered
  • Form
    Liquid
  • Additional notes
    Working aliquots are stable for up to 3 months when stored at -20°C. Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

General Info

  • Alternative names
    • Adipocyte differentiation inhibitor protein
    • Brevideltin
    • Delta like 1 homolog
    • Delta like homolog
    • Delta like protein
    • Delta1
    • DLK
    • DLK 1
    • DLK-1
    • DLK1
    • DLK1_HUMAN
    • FA 1
    • FA1
    • Fetal antigen 1
    • pG2
    • Preadipocyte factor 1
    • Pref
    • Pref 1
    • Pref1
    • Protein delta homolog 1
    • Secredeltin
    • ZOG
    see all
  • Function
    May have a role in neuroendocrine differentiation.
  • Tissue specificity
    Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
  • Sequence similarities
    Contains 6 EGF-like domains.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt

Images

  • Induction of SOX-9 with the treatment of recombinant mouse DLK-Fc (ab108574).
    A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5µg/ml of ab108574 as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-rabbit Sox-9 and anti-rabbit GAPDH.

    1=0 min; 2=10 min; 3=30 min; 4=1 h; 5=2 h; 6=3 h; 7=4 h; 8=24 h.

     

References

ab108574 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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