Recombinant mouse Eph receptor B1 protein (ab56257)

Overview

Description

  • NatureRecombinant
  • SourceBaculovirus infected Sf9 cells
  • Amino Acid Sequence
    • SpeciesMouse
    • Amino acids591 to 984

Associated products

Specifications

Our Abpromise guarantee covers the use of ab56257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 90 % SDS-PAGE.
    Source: Sf9 insect cells.
  • FormLiquid
  • Additional notes

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.1mM EDTA, 0.1mM PMSF, 0.1mM DTT, 0.1mM EGTA, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Cek 6
    • EK6
    • ELK
    • Elkh
    • EPH receptor B1
    • Eph tyrosine kinase 2
    • EPH-like kinase 6
    • Ephb1
    • EPHB1_HUMAN
    • Ephrin type B receptor 1
    • Ephrin type-B receptor 1
    • EPHT2
    • HEK 6
    • HEK6
    • NET
    • Neuronally-expressed EPH-related tyrosine kinase
    • soluble EPHB1 variant 1
    • Tyrosine protein kinase receptor EPH 2
    • Tyrosine-protein kinase receptor EPH-2
    see all
  • FunctionReceptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. Binding with the guidance cue ephrin-B2 at the optic chiasm midline redirect ventrotemporal (VT) retinal ganglion cells (RGCs) axons ipsilaterally. May be involved in cell-cell interactions in the nervous system.
  • Tissue specificityPreferentially expressed in brain.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localizationMembrane.
  • Information by UniProt

Recombinant mouse Eph receptor B1 protein images

  • Kinase Assay Activity Plot.

    ab56257 at 0.1µg/µl.

    Specific activity determined as 143 nmol/min/mg.

  • ab56257 on SDS-PAGE, MW 62kDa.

References for Recombinant mouse Eph receptor B1 protein (ab56257)

ab56257 has not yet been referenced specifically in any publications.

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