The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. ab50235 purity is greater than 98% by SDS-PAGE and HPLC analyses.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. Repeated freeze thaw cycles will result in loss of activity.
Basic fibroblast growth factor
Basic fibroblast growth factor bFGF
Fibroblast growth factor 2
Fibroblast growth factor 2 (basic)
Fibroblast growth factor, basic
Heparin binding growth factor 2 precursor
Heparin-binding growth factor 2
FunctionPlays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
Tissue specificityExpressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
Post-translational modificationsPhosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
Cellular localizationSecreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.