Recombinant mouse GM-CSF protein (ab9742)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesMouse
    • SequenceMAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK
    • Molecular weight14 kDa

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The ED50 as determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

  • Applications

    Functional Studies

    SDS-PAGE

  • FormLyophilised
  • Additional notesThe ED50, determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells, is < 0.05 ng/ml, corresponding to a specific activity of > 2 x 107 units/mg.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Colony stimulating factor
    • Colony Stimulating Factor 2
    • Colony stimulating factor 2 (granulocyte-macrophage)
    • Colony-stimulating factor
    • CSF
    • CSF 2
    • CSF2
    • CSF2_HUMAN
    • GM-CSF
    • GMCSF
    • Granulocyte Macrophage Colony Stimulating Factor
    • Granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • MGI1GM
    • Molgramostin
    • Pluripoietin-a
    • Sargramostim
    see all
  • FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • Sequence similaritiesBelongs to the GM-CSF family.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant mouse GM-CSF protein (ab9742)

ab9742 has not yet been referenced specifically in any publications.

Product Wall

Application Functional Studies
this protein worked fine for the differentiation of mouse bone marrow-derived macrophage cells with lentivirus transduction. we checked its purity via SDS-PAGE as well prior to the experiment, QC was good.
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Abcam user community

Verified customer

Submitted Oct 12 2016

Abreviews
Application Other
We used this rGM-CSF in cell culture to differentiate murine bone marrow cells into bone marrow-derived dendritic cells (BMDCs), and we had a much better yield than when using a competitor's product. In addition, after selection by magnetic beads (negative selection for F4/80, then positive selection for CD11c), we had >90% purity of BMDCs using this rGM-CSF for culture. Finally, using the cells generated from this rGM-CSF, we saw significant anti-fungal activity of these BMDCs in vitro against Cryptococcus neoformans.
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Ms. Cheryl Sandoval

Verified customer

Submitted Jun 23 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"