The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityDetermined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25°C, is greater than 750 nM/min per µg of enzyme.
Endotoxin level< 0.100 Eu/µg
% SDS-PAGE. Greater than 98% by SDS-PAGE and HPLC analyses.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
Cathepsin G like 1
Cathepsin G-like 1
Cytotoxic serine protease B
Cytotoxic T lymphocyte associated serine esterase 1
Cytotoxic T lymphocyte proteinase 2
Cytotoxic T-lymphocyte proteinase 2
Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1)
Human lymphocyte protein
Protease, serine, B
T cell serine protease 1-3E
T-cell serine protease 1-3E
FunctionThis enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Sequence similaritiesBelongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.
Cellular localizationCytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.