Recombinant mouse Hsp60 protein (ab92364)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant mouse Hsp60 protein
See all Hsp60 proteins and peptides -
Biological activity
ATPase Activity Assay: Positive
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Purity
> 90 % SDS-PAGE.
>90% pure as determined by SDS-PAGE and Western blot analyses. This protein does not contain E. coli GroEL as demonstrated by western blot analysis. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab92364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.158% Tris HCl, 0.0292% EDTA, 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 60 kDa chaperonin
- 60 kDa heat shock protein, mitochondrial
- CH60_HUMAN
see all -
Function
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
Involvement in disease
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
Sequence similarities
Belongs to the chaperonin (HSP60) family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
Images
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SDS-PAGE Analysis:
Lane 1: Molecular weight markers
Lane 2: ab92364 at 2.0 μg -
All lanes : Hsp60 monoclonal antibody at 1 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : ab92364 at 0.1 µg
Lane 3 : E. coli GroEL Protein at 0.1 µg -
All lanes : GroEL monoclonal antibody at 1 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : ab92364 at 0.1 µg
Lane 3 : E. coli GroEL Protein at 0.1 µg
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab92364 has been referenced in 1 publication.
- Zhang H et al. Muscone Inhibits the Excessive Inflammatory Response in Myocardial Infarction by Targeting TREM-1. Evid Based Complement Alternat Med 2022:9112479 (2022). PubMed: 35591864