Recombinant mouse Hsp70 protein (ab113187)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Mouse
    • Molecular weight
      70 kDa
    • Amino acids
      1 to 641

Specifications

Our Abpromise guarantee covers the use of ab113187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    ATPase activity assay (positive) but there is not quantification of activity
  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Purity
    > 85 % SDS-PAGE.
    ab113187 is purified by multi-step chromatography.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.15% Glutathione, 99% PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • DnaK type molecular chaperone HSP70 1
    • Epididymis secretory protein Li 103
    • FLJ54303
    • FLJ54370
    • FLJ54392
    • FLJ54408
    • FLJ75127
    • Heat shock 70 kDa protein 1
    • Heat shock 70 kDa protein 1/2
    • Heat shock 70 kDa protein 1A/1B
    • Heat shock 70kDa protein 1A
    • Heat shock 70kDa protein 1B
    • Heat shock induced protein
    • HEL S 103
    • HSP70 1
    • HSP70 1B
    • HSP70 2
    • HSP70-1/HSP70-2
    • HSP70-1A
    • HSP70.1
    • HSP70.1/HSP70.2
    • HSP70I
    • HSP71_HUMAN
    • HSP72
    • HSPA1
    • HSPA1A
    • HSPA1B
    see all
  • Function
    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
  • Tissue specificity
    HSPA1B is testis-specific.
  • Sequence similarities
    Belongs to the heat shock protein 70 family.
  • Cellular localization
    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

Images

  • SDS-PAGE showing ab113187 (1µg).
  • Lane 1: MWM
    Lane 2: 100 ng ab113187
    Lane 3: 50 ng E. coli DnaK Protein

    Left: probed with Hsp70 mAb at 1.0 µg/ml.
    Right: probed with DnaK mAb at 1.0 µg/ml.

References

ab113187 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

This protein, ab113187, does not have a tag.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up