The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Assay 1: Determined by its ability to induce cell proliferation in T-cell enriched PBMCs. The expected ED50 is ≤ 0.1 ng/mL, corresponding to a specific activity of ≥ 1 x 107 units/mg. Assay 2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is ≤ 0.1ng/mL, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Endotoxin level< 1.000 Eu/µg
% SDS-PAGE. Endotoxin level is < 0.1 ng/µg of protein (<1 EU/µg).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Lyophilized from 1x PBS.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge vial prior to opening. Reconstitute in 1x PBS, pH 7.2-7.4 to 0.1-1.0 mg/mL. Do not vortex.
Store at 2°C to 8°C for 1 week, or further dilute in a buffer containing a carrier protein (example 0.1% BSA) for extended storage.
FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Sequence similaritiesBelongs to the IL-6 superfamily.