Overview

Description

  • Nature
    Recombinant
  • Source
    CHO cells
  • Amino Acid Sequence
    • Species
      Mouse
    • Sequence
      -p35 subunit --RVIPVSGP ARCLSQSRNL LKTTDDMVKT AREKLKHYSC TAEDIDHEDI TRDQTSTLKT CLPLELHKNE SCLATRETSS TTRGSCLPPQ KTSLMMTLCL GSIYEDLKMY QTEFQAINAA LQNHNHQQII LDKGMLVAID ELMQSLNHNG ETLRQKPPVG EADPYRVKMK LCILLHAFST RVVTINRVMG YLSSA -p40 subunit --MWELEKDV YVVEVDWTPD APGETVNLTC DTPEEDDITW TSDQRHGVIG SGKTLTITVK EFLDAGQYTC HKGGETLSHS HLLLHKKENG IWSTEILKNF KNKTFLKCEA PNYSGRFTCS WLVQRNMDLK FNIKSSSSSP DSRAVTCGMA SLSAEKVTLD QRDYEKYSVS CQEDVTCPTA EETLPIELAL EARQQNKYEN YSTSFFIRDI IKPDPPKNLQ MKPLKNSQVE VSWEYPDSWS TPHSYFSLKF FVRIQRKKEK MKETEEGCNQ KGAFLVEKTS TEVQCKGGNV CVQAQDRYYN SSCSKWACVP CRVRS

Associated products

Specifications

Our Abpromise guarantee covers the use of ab50153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Assay 1: Determined by its ability to induce cell proliferation in T-cell enriched PBMCs. The expected ED50 is ≤ 0.1 ng/mL, corresponding to a specific activity of ≥ 1 x 107 units/mg.
    Assay 2: Determined by its ability to stimulate the proliferation of 2D6 cells. The expected ED50 is ≤ 0.1ng/mL, corresponding to a specific activity of ≥ 1 x 107 units/mg.

  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    > 98 % SDS-PAGE.
    Endotoxin level is < 0.1 ng/µg of protein (<1 EU/µg).
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.2

    Lyophilized from 1x PBS.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge vial prior to opening. Reconstitute in 1x PBS, pH 7.2-7.4 to 0.1-1.0 mg/mL. Do not vortex. Store at 2°C to 8°C for 1 week, or further dilute in a buffer containing a carrier protein (example 0.1% BSA) for extended storage.

General Info

  • Alternative names
    • CLMF
    • CLMF p35
    • CLMF p40
    • CLMF1
    • CLMF2
    • Cytotoxic lymphocyte maturation factor 1, p35
    • Cytotoxic lymphocyte maturation factor 2
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • IL 2 subunit p40
    • IL-12 subunit p35
    • IL-12A
    • IL-12B
    • IL12, p35 subunit
    • IL12, subunit p40
    • IL12A
    • IL12A_HUMAN
    • IL12B
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 beta chain
    • Interleukin 12 subunit beta
    • Interleukin 12, p40
    • Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
    • Interleukin 12B
    • Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1, 35 kD subunit
    • natural killer cell stimulatory factor 2
    • Natural killer cell stimulatory factor, 40 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NK cell stimulatory factor chain 2
    • NKSF
    • NKSF1
    • NKSF2
    • P35
    see all
  • Function
    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab50153 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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