Recombinant mouse IL-17F protein (Animal Free) (ab207953)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.050 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant mouse IL-17F protein (Animal Free)
See all IL-17F proteins and peptides -
Biological activity
The activity is determined by the dose-dependant induction of IL-6 in NIH 3T3 cells and is typically 35-50 ng/mL. This corresponds to an expected specific activity of 2.8 x 104 units/mg.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 0.050 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Nature
Recombinant -
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Species
Mouse -
Sequence
MRKNPKAGVP ALQKAGNCPP LEDNTVRVDI RIFNQNQGIS VPREFQNRSS SPWDYNITRD PHRFPSEIAE AQCRHSGCIN AQGQEDSTMN SVAIQQEILV LRREPQGCSN SFRLEKMLLK VGCTCVKPIV HQAA -
Predicted molecular weight
30 kDa -
Amino acids
29 to 161 -
Additional sequence information
This product is for the mature full length protein. The signal peptide is not included.
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Specifications
Our Abpromise guarantee covers the use of ab207953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.1% Trifluoroacetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile water to 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. Dilute to working aliquots in a 0.1% BSA solution.
General Info
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Alternative names
- CANDF6
- Cytokine ML 1
- Cytokine ML-1
see all -
Function
Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation. -
Tissue specificity
Expressed in activated, but not resting, CD4+ T-cells and activated monocytes. -
Involvement in disease
Candidiasis, familial, 6 -
Sequence similarities
Belongs to the IL-17 family. -
Cellular localization
Secreted. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab207953 has not yet been referenced specifically in any publications.