The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThe biological activity was determined by measuring the dose dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100 ng/mL of mouse IL20.
% SDS-PAGE. ab73330 was purified by proprietary chromatographic techniques.
Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in sterile 18MOhm/cm H2O to not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Four alpha helix cytokine
FunctionCytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.
Tissue specificityExpressed at very low levels in skin, trachea, and other tissues.