Recombinant mouse IL27-A protein (Animal Free) (ab187220)


  • Product name
    Recombinant mouse IL27-A protein (Animal Free)
  • Protein length
    Full length protein


  • Nature
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
    • Sequence
    • Molecular weight
      24 kDa
    • Amino acids
      29 to 234


Our Abpromise guarantee covers the use of ab187220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The activity is measured via dose-dependent inhibition of IL17A expression from mouse CD4+ splenocytes stimulated with TGF-β and IL6. 50ng/mL typically inhibits greater than 25% of IL17A expression.

  • Applications

    Functional Studies


  • Endotoxin level
    <=1.000 Eu/µg
  • Purity
    >= 97 % SDS-PAGE.
    Determined by reducing and Non-reducing SDS-PAGE
  • Form
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 7.4
    Constituent: 0.14% Sodium phosphate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

General Info

  • Alternative names
    • EBI3
    • IL 27
    • IL 27 p28 subunit
    • IL 27A
    • IL 30
    • IL-27 subunit alpha
    • IL-27-A
    • IL27
    • IL27-A
    • IL27A
    • IL27A_HUMAN
    • IL27p28
    • IL30
    • Interleukin 27
    • Interleukin 27 subunit alpha
    • Interleukin 27, 28-KD subunit
    • Interleukin 30
    • Interleukin-27 subunit alpha
    • p28
    see all
  • Function
    Cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation.
  • Tissue specificity
    Expressed in monocytes and in placenta.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
  • Cellular localization
    Secreted. Does not seem to be secreted without coexpression of EBI3.
  • Information by UniProt


ab187220 has not yet been referenced specifically in any publications.

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