The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological Activity: The ED50 as determined by a cytopathic affect inhibition assay with murine NIH 3T3 cells challenged with VSV virus is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
% SDS-PAGE. Purity by SDS-PAGE and HPLC: 95%
Endotoxin level is <0.1 ng per µg of IFN gamma.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitute in ddH2O to a concentration of 1 mg/ml. Further dilutions should be made into RPMI + 10% FCS and stored at 4°C for 1 week or –20°C for future use.
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Released primarily from activated T lymphocytes.
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Belongs to the type II (or gamma) interferon family.
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.