Recombinant mouse Interferon gamma protein (ab123747)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP01580
    • SpeciesMouse
    • Molecular weight16 kDa
    • Amino acids23 to 155

Specifications

Our Abpromise guarantee covers the use of ab123747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityBiological Activity: The ED50 as determined by a cytopathic affect inhibition assay with murine NIH 3T3 cells challenged with VSV virus is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Purity> 95 % SDS-PAGE.
    Purity by SDS-PAGE and HPLC: 95% Endotoxin level is <0.1 ng per µg of IFN gamma.
  • FormLyophilised
  • Additional notesEndotoxin Level: <0.1 ng/µg.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    pH: 7.00
    Constituents: 0.16% Sodium phosphate, 0.15% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in ddH2O to a concentration of 1 mg/ml. Further dilutions should be made into RPMI + 10% FCS and stored at 4°C for 1 week or –20°C for future use.

General Info

  • Alternative names
    • IFG
    • IFI
    • IFN gamma
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    see all
  • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificityReleased primarily from activated T lymphocytes.
  • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant mouse Interferon gamma protein (ab123747)

ab123747 has not yet been referenced specifically in any publications.

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