Recombinant mouse Interferon gamma protein (ab9922)

Overview

Description

  • NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • SpeciesMouse
    • SequenceRecombinant Murine IFN-g is a 15.6 kDa protein containing 134 amino acid residues: MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLR LFEVLKDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV N NPQVQRQAF NELIRVVHQLLPESSLRKRK RSRC

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level< 0.100 Eu/µg
  • FormLyophilised
  • Additional notesThe ED50 is <0.2ng/ml, determined by ability to inhibit proliferation of murine WEHI-279 cells, corresponding to a specific activity of >5 x 106 units/mg
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionPlease reconstitute this product in 5-10mM Sodium Phosphate, pH 8.0 to a concentration of 1.0mg/ml.

General Info

  • Alternative names
    • IFG
    • IFI
    • IFN gamma
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    see all
  • FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificityReleased primarily from activated T lymphocytes.
  • Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similaritiesBelongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications
    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localizationSecreted.
  • Information by UniProt

References for Recombinant mouse Interferon gamma protein (ab9922)

ab9922 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Functional Studies
We used the recombinant IFN-g protein in cell culture. It reconstituted easily, and worked well in vitro to stimulate cells.
Username

Ms. Cheryl Sandoval

Verified customer

Submitted Sep 13 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"