Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Species
      Mouse

Associated products

Specifications

Our Abpromise guarantee covers the use of ab60304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific activity: 124 nmol/min/mg. (lot K110-1) Specific activity: 92 nmol/min/mg. (lot C341-2)
  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

    Other

  • Form
    Liquid
  • Additional notes

    ab43598 (Human ATF2 protein fragment) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • AI849689
    • c Jun N terminal kinase 1
    • C-JUN kinase 1
    • c-Jun N-terminal kinase 1
    • EC 2.7.11.24
    • JNK
    • JNK 1
    • JNK-46
    • JNK1A2
    • JNK21B1/2
    • MAP kinase 8
    • MAPK 8
    • mapk8
    • Mitogen activated protein kinase 8
    • Mitogen-activated protein kinase 8
    • MK08_HUMAN
    • p54 gamma
    • Prkm8
    • Protein kinase JNK1
    • Protein kinase, mitogen-activated, 8
    • SAPK 1
    • SAPK gamma
    • SAPK1
    • Stress-activated protein kinase 1
    • Stress-activated protein kinase JNK1
    see all
  • Function
    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt

Images

  • Sample Kinase assay plot.
  • ab60304 on SDS-PAGE.

References

This product has been referenced in:
  • Prakash A  et al. Phosphorylation Sites Identified in the NEIL1 DNA Glycosylase Are Potential Targets for the JNK1 Kinase. PLoS One 11:e0157860 (2016). Read more (PubMed: 27518429) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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