Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Mouse
    • Molecular weight
      71 kDa including tags
    • Amino acids
      1 to 464

Specifications

Our Abpromise guarantee covers the use of ab119734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Specific Activity: 35 nmol/min/mg
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 80 % SDS-PAGE.
    Purity determined to be >80% by densitometry.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 25% Glycerol, 0.004% DTT, 0.003% EDTA, 0.31% Glutathione, 0.002% PMSF, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • c Jun kinase 3
    • c-Jun N-terminal kinase 3
    • cJun N terminal kinase 3
    • FLJ12099
    • FLJ33785
    • JNK3
    • JNK3 alpha protein kinase
    • JNK3A
    • MAP kinase
    • MAP kinase 10
    • MAP kinase p49 3F12
    • MAPK 10
    • Mapk10
    • MGC50974
    • mitogen activated protein kinase 10
    • Mitogen-activated protein kinase 10
    • MK10_HUMAN
    • p493F12
    • p54bSAPK
    • PRKM10
    • protein kinase mitogen activated 10
    • SAPK1b
    • Stress activated protein kinase 1b
    • stress activated protein kinase beta
    • Stress activated protein kinase JNK3
    • Stress-activated protein kinase JNK3
    see all
  • Function
    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity.
  • Tissue specificity
    Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
  • Involvement in disease
    Defects in MAPK10 are a cause of epileptic encephalopathy Lennox-Gastaut type (EELG) [MIM:606369]. Epileptic encephalopathies of the Lennox-Gastaut group are childhood epileptic disorders characterized by severe psychomotor delay and seizures. Note=A chromosomal aberration involving MAPK10 has been found in a single patient. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt

Images

  • The specific activity of ab119734 was determined to be 35 nmol/min/mg.
  • SDS-PAGE analysis of ab119374.

References

ab119734 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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