The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab60014 (Mouse MYL9 full length protein) can be utilized as a substrate for the following active protein Kinases:
ab55674 (Active human Myosin light chain kinase protein fragment) ab84799 (Active human MYLK3 full length protein) ab84800 (Active human MYLK2 full length protein) ab89742 (Active human DAP Kinase 2 full length protein) ab190995 (Active human MYLK4 full length protein)
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
FunctionMyosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Tissue specificitySmooth muscle tissues and in some, but not all, nonmuscle cells.
Sequence similaritiesContains 3 EF-hand domains.
Post-translational modificationsPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.