Recombinant Mouse PAI1 + PLAT protein (ab92709)

Overview

Description

  • Nature
    Recombinant
  • Source
    Insect cells
  • Amino Acid Sequence
    • Species
      Mouse

Specifications

Our Abpromise guarantee covers the use of ab92709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    Mouse sc-tPA is reacted with an excess of Mouse PAI1 stable mutant at physiological pH. The resulting 1:1 molar complex is purified from the remaining PAI1 by affinity chromatography with immobilized antibodies to Mouse tPA.
  • Form
    Liquid
  • Additional notes
    Solubility: > 2 mg/mL
    Spectrophotometric Data: ε0.1%=1.5
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.4M HEPES, 0.1M Sodium chloride, pH 7.4

General Info

  • Alternative names
    • Alteplase
    • PAI 1
    • PLANH1
    • Plasminogen activator inhibitor 1
    • PLAT
    • Reteplase
    • t PA
    • t plasminogen activator
    • tPA
    see all
  • Relevance
    PAI1 acts as "bait" for tissue plasminogen activator (TPA), urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. TPA: This converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events.
  • Cellular localization
    Secreted protein; extracellular space.

Images

  • 10% SDS-PAGE
    Lane 1: Mouse PAI1, Non-reduced
    Lane 2: Mouse tPA, Non-reduced
    Lane 3: Mouse PAI1 + tPA, Non-reduced
    Lane 4: Mouse PAI1 + tPA, Reduced
    Lane 5: Mouse PAI1, Reduced
    Lane 6: Mouse tPA, Reduced
    Lane 7: Prestained standard

References

ab92709 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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