Recombinant mouse PCSK9 protein (ab167759)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 97% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
Description
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Product name
Recombinant mouse PCSK9 protein
See all PCSK9 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized Human LDL receptor protein (ab220582) at 10 μg/mL (100 μL/well) can bind ab167759 with a linear range of 0.125-1 μg/mL.
Measured by its binding ability in a BLI assay. Loaded Recombinant human LDL Receptor protein (ab220570) on Protein A Biosensor, can bind Recombinant mouse PCSK9 protein (ab167759) with an affinity constant of 2.28 nM as determined in BLI assay.
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Purity
> 97 % SDS-PAGE.
ab167759 is lyophilized from 0.22 µm filtered solution. Purified by Immobilized metal affinity chromatography. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
QDEDGDYEELMLALPSQEDGLADEAAHVATATFRRCSKEAWRLPGTYIVV LMEETQRLQIEQTAHRLQTRAARRGYVIKVLHIFYDLFPGFLVKMSSDLL GLALKLPHVEYIEEDSFVFAQSIPWNLERIIPAWHQTEEDRSPDGSSQVE VYLLDTSIQGAHREIEGRVTITDFNSVPEEDGTRFHRQASKCDSHGTHLA GVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLIQPSG PLVVLLPLAGGYSRILNAACRHLARTGVVLVAAAGNFRDDACLYSPASAP EVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCFMS QSGTSQAAAHVAGIVARMLSREPTLTLAELRQRLIHFSTKDVINMAWFPE DQQVLTPNLVATLPPSTHETGGQLLCRTVWSAHSGPTRTATATARCAPEE ELLSCSSFSRSGRRRGDWIEAIGGQQVCKALNAFGGEGVYAVARCCLVPR ANCSIHNTPAARAGLETHVHCHQKDHVLTGCSFHWEVEDLSVRRQPALRS RRQPGQCVGHQAASVYASCCHAPGLECKIKEHGISGPSEQVTVACEAGWT LTGCNVLPGASLTLGAYSVDNLCVARVHDTARADRTSGEATVAAAICCRS RPSAKASWVQ -
Predicted molecular weight
72 kDa including tags -
Amino acids
35 to 694 -
Tags
His tag C-Terminus -
Additional sequence information
NCBI Accession number: AAH38085.1
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab167759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
ELISA
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 94% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
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Alternative names
- Convertase subtilisin/kexin type 9 preproprotein
- FH3
- HCHOLA3
see all -
Function
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. -
Tissue specificity
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. -
Involvement in disease
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant. -
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain. -
Post-translational
modificationsThe soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. -
Cellular localization
Secreted. - Information by UniProt
Images
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Immobilized Human LDL receptor protein (ab220582) at 5 μg/mL (100 μL/well) can bind Recombinant mouse PCSK9 protein (ab167759) with a linear range of 0.078-0.313 μg/mL.
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Immobilized Human LDL receptor protein (ab220582) at 10 μg/mL (100 μL/well) can bind ab167759 with a linear range of 0.125-1 μg/mL.
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SDS-PAGE analysis of reduced ab167759 stained overnight with Coomassie Blue. The protein migrates as 17 kDa and 65 kDa under reducing (R) condition due to glycosylation and proteolytic digestion.
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Loaded Recombinant human LDL Receptor protein (ab220570) on Protein A Biosensor, can bind Recombinant mouse PCSK9 protein (ab167759) with an affinity constant of 2.28 nM as determined in BLI assay.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab167759 has been referenced in 2 publications.
- Kawakami R et al. Development of vaccine for dyslipidemia targeted to a proprotein convertase subtilisin/kexin type 9 (PCSK9) epitope in mice. PLoS One 13:e0191895 (2018). PubMed: 29438441
- Sun H et al. PCSK9 deficiency reduces atherosclerosis, apolipoprotein B secretion, and endothelial dysfunction. J Lipid Res 59:207-223 (2018). PubMed: 29180444