Recombinant mouse PDE10A protein (ab79743)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant mouse PDE10A protein
See all PDE10A proteins and peptides -
Biological activity
Specific Activity: 599 pmol/min/µg.
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Purity
> 80 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse
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Specifications
Our Abpromise guarantee covers the use of ab79743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 5''-cyclic phosphodiesterase 10A
- cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A
- cAMP and cAMP-inhibited cGMP 3''
see all -
Function
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. -
Tissue specificity
Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum. -
Pathway
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
Domain
The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region. -
Cellular localization
Cytoplasm. Located mostly to soluble cellular fractions. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab79743 has not yet been referenced specifically in any publications.