Recombinant Mouse Procalcitonin protein (ab203548)
Key features and details
- Expression system: Escherichia coli
- Purity: > 94% Densitometry
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: WB, MS, SDS-PAGE, ELISA
Description
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Product name
Recombinant Mouse Procalcitonin protein
See all Procalcitonin proteins and peptides -
Purity
> 94 % Densitometry. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MKHHHHHHAS VPLRSILESS PGMATLSEEE VRLLAALVQD YMQMKARELE QEEEQEAEGS SLDSPRSKRC GNLSTCMLGT YTQDLNKFHT FPQTSIGVEA PGKKRDVAKD LETNHQSHFG N -
Predicted molecular weight
14 kDa including tags -
Amino acids
26 to 136 -
Tags
His tag N-Terminus -
Additional sequence information
This is the full length protein without the signal peptide.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab203548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Mass Spectrometry
SDS-PAGE
ELISA
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Mass spectrometry
LC-MS/MS -
Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.24% Tris buffer, 0.29% Sodium chloride
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ReconstitutionAdd 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
General Info
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Alternative names
- CALC1
- CALCA
- calcitonin related polypeptide alpha
see all -
Relevance
Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis. -
Cellular localization
Secreted
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab203548 has not yet been referenced specifically in any publications.