The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityOne unit of protease hydrolyzes 1 picomole of Mouse renin FRET substrate 5-FAM/QXLTM 520 per minute at pH 7.5 at 25° C.
One unit of protease hydrolyzes 1 picomole of Mouse renin FRET substrate 5-FAM/QXLTM 520 per minute at pH 7.5 at 25° C.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituent: 0.6% Tris
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Angiotensin forming enzyme
Renin precursor renal
FunctionRenin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Involvement in diseaseDefects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
Sequence similaritiesBelongs to the peptidase A1 family.
Cellular localizationSecreted. Membrane. Associated to membranes via binding to ATP6AP2.