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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Mouse
    • Amino acids
      1 to 321
    • Tags
      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab42574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of mouse PTP1B was determined using pNPP. Enzyme reaction condition: 20 mM pNPP, 5 min incubation at 30C, 2 ug/ml enzyme.
  • Applications

    Inhibition Assay

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 75mM Sodium chloride, 25mM Tris HCl, 10mM Glutathione, 1mM DTT, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • PTP1B
    • Non receptor tyrosine phosphatase 1
    • Protein phosphotyrosylphosphatase 1B
    • Protein tyrosine phosphatase 1B
    • Protein tyrosine phosphatase non receptor type 1
    • Protein tyrosine phosphatase placental
    • Protein-tyrosine phosphatase 1B
    • PTN1_HUMAN
    • PTP 1B
    • PTP-1B
    • PTPN 1
    • PTPN1
    • Tyrosine protein phosphatase non receptor type 1
    • Tyrosine-protein phosphatase non-receptor type 1
    see all
  • Function
    May play an important role in CKII- and p60c-src-induced signal transduction cascades.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt

References for Recombinant mouse PTP1B protein (ab42574)

ab42574 has not yet been referenced specifically in any publications.

Publishing research using ab42574? Please let us know so that we can cite the reference in this datasheet.

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