• NatureRecombinant
  • SourceEscherichia coli
  • Amino Acid Sequence
    • AccessionP20826
    • SpeciesMouse
    • Molecular weight18 kDa
    • Amino acids26 to 190
    • Additional sequence informationMurine SCF is a 18.3 kDa protein containing 164 amino acid residues, as the soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.

Associated products


Our Abpromise guarantee covers the use of ab106309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityThe ED50 as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cell line is < 10 ng/ml, corresponding to a specific activity of >1 x 105 units/mg.
  • Applications


    Functional Studies

  • Endotoxin level< 0.100 Eu/µg
  • Purity> 98 % SDS-PAGE.

  • FormLyophilised
  • Additional notes

    Endotoxin level: < 0.1 ng/g of SCF


  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 30mM Tris, 100mM Sodium chloride, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionReconstitute in 50 mM acetic acid or water to a concentration of 0.1 mg/ml. Do not vortex

General Info

  • Alternative names
    • C kit ligand
    • C-kit ligand
    • Ckit ligand
    • DCUA
    • DFNA69
    • DKFZp686F2250
    • familial progressive hyperpigmentation 2
    • FPH2
    • FPHH
    • KIT ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • MGF stem cell factor
    • SCF
    • SF
    • SHEP7
    • sKITLG
    • Soluble KIT ligand
    • Steel factor
    • steel, mouse, homolog of
    • Stem cell factor
    • Stem cell factor precursor
    see all
  • FunctionLigand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • Involvement in diseaseHyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • Sequence similaritiesBelongs to the SCF family.
  • Developmental stageActs in the early stages of hematopoiesis.
  • Post-translational
    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • Cellular localizationSecreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt

Recombinant mouse SCF protein images

  • 15% SDS-PAGE.
    Lane 1: Molecular weight marker.
    Lane 2: SCF protein.
    Visualised by silver stain.

References for Recombinant mouse SCF protein (ab106309)

ab106309 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab106309.
Please use the links above to contact us or submit feedback about this product.